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Kinyoun stain : ウィキペディア英語版 | Kinyoun stain The Kinyoun method, or Kinyoun stain, is an acid-fast procedure used to stain any species of the genus ''Mycobacterium'' and ''Nocardia〔Geo F. B et al, Jawetz, Melnick and Adelberg's Medical Microbiology, 25th Edition, Lange Medical,2004, page 182〕'' species. It involves the application of a primary stain (carbol fuchsin), a decolorizer (acid-alcohol), and a counterstain (methylene blue).〔Hussey, A. M., Zayaitz, A., ("Acid-Fast Stain Protocols" ), ''American Society for Microbiology'', 8 September 2008. Retrieved on 1 November 2014.〕 Unlike the Ziehl-Neelson stain (Z-N stain), the Kinyoun method of staining does not require heating.〔Murray PR, Baron E, Pfaller M, Tenover F, Yolken, Eds. Manual of clinical microbiology. 7th ed. Washington, DC: ASM, 1999.〕〔Baron EJ, Finegold SM. Bailey and Scott's diagnostic microbiology. 8th ed. St. Louis: Mosby, 1990.〕 ==See also==
* Auramine-rhodamine stain
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